Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

by

The present CoVid-19 disaster is revealing the strengths and the weaknesses of the world’s capability to reply to a worldwide well being disaster. A crucial weak spot has resulted from the extreme centralization of the present biomanufacturing capacities, a matter of nice concern, if not a supply of nationalistic tensions. On the constructive facet, scientific knowledge and knowledge have been shared at an unprecedented pace fuelled by the preprint phenomena, and this has significantly strengthened our skill to develop new technology-based options. In this work, we discover how, in a context of fast alternate of scientific info, plant biofactories can function a fast and simply adaptable answer for native manufacturing of bioreagents, extra particularly recombinant antibodies.

For this objective, we examined our skill to provide, in the framework of an tutorial lab and in a matter of weeks, milligram quantities of six totally different recombinant monoclonal antibodies in opposition to SARS-CoV-2 in Nicotiana benthamiana. For the design of the antibodies, we took benefit, amongst different knowledge sources, of the DNA sequence info made quickly available by different teams in preprint publications. mAbs have been engineered as single-chain fragments fused to a human gamma Fc and transiently expressed utilizing a viral vector. In parallel, we additionally produced the recombinant SARS-CoV-2 N protein and the receptor binding area (RBD) of the Spike protein in planta and used them to check the binding specificity of the recombinant mAbs.

Finally, for two of the antibodies, we assayed a easy scale-up manufacturing protocol based mostly on the extraction of apoplastic fluid. Our outcomes point out that gram quantities of anti-SARS-CoV-2 antibodies could possibly be simply produced in little greater than 6 weeks in repurposed greenhouses with little infrastructure necessities utilizing N. benthamiana as manufacturing platform. Similar procedures could possibly be simply deployed to provide diagnostic reagents and, finally, could possibly be tailored for fast therapeutic responses.

To determine tutorial elements pertaining to varsity college students, calendar timing, and explicit moments which are uniquely related to elevated probability of prescription stimulant misuse (intentions and precise conduct) in each day life. Participants have been 297 freshmen and sophomores at a big public college in the United States in 2017-2019. Participants accomplished survey measures throughout lab visits and ecological momentary evaluation procedures in each day life.

Hypotheses have been examined utilizing multilevel fashions that accounted for the nested knowledge and demographic covariates. Student, calendar-based, and momentary tutorial elements have been uniquely related to stimulant misuse intentions in each day life. Real-time tutorial occasions, past the proportion of tutorial occasions skilled, emerged as a strong predictor of misuse conduct. Within-person hyperlinks between real-time momentary predictors and misuse conduct have been moderated by finals week timing. Findings provide implications for prevention and intervention methods for faculty college students in danger for prescription drug misuse.

Bringing Real-World Microbiology Experiences to Undergraduate Students in Resource-Limited Environments

Undergraduate microbiology curriculum must be amenable to periodic modifications to include new developments and concepts. The curriculum must be used not merely as a strategy to disseminate information but additionally as a strategy to permit college students to expertise the method of science. In the context of undergraduate microbiology training in Osmania University (Hyderabad, India), current curriculum doesn’t explicitly permit college students to interact in deeper understanding of ideas and understanding of the method of science, each in lecture and laboratory programs.

The evaluation strategies which are at the moment used are restricted in scope as they solely take a look at factual recall and superficial understanding of the topic and really minimally assess crucial pondering abilities. Another issue hampering innovation in the broader context of undergraduate training is the unavailability and inaccessibility to enough sources. To handle the difficulty of resource-limitations in implementing actions that expose undergraduate college students to real-world microbiology experiences, a collaboration between a analysis institute and two educating faculties was fashioned.

This collaboration concerned trainer and scholar workshops on exploring microbial range utilizing 16S rRNA evaluation with a view of mixing novel analysis questions with technical abilities in the undergraduate microbiology lab. This effort is an instance of educators offering college students with genuine experiences and, serving to them achieve crucial data and analysis abilities in microbiology even beneath useful resource constraints, and college students demonstrating motivation to take part in related actions in the long run. The collaborative effort described right here is usually a broadly sustainable mannequin to enhance general undergraduate training in comparatively resource-limited environments.

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Integrating STEM in elementary lecture rooms utilizing model-eliciting actions: responsive skilled improvement for arithmetic coaches and lecturers.

This analysis highlights a school-university collaboration to pilot an expert improvement framework for integrating STEM in Okay-6 arithmetic lecture rooms in a mid-Atlantic suburban faculty division. Because arithmetic inside STEM integration is commonly characterised because the calculations or the information representations in science lecture rooms, know-how labs, or outside-of-school packages, growing a cheap and real looking conceptualization of STEM integration for arithmetic lecturers and coaches could also be particularly difficult.

Using design-based implementation analysis, college facilitators labored with eight arithmetic lecturers and coaches to assemble an accessible imaginative and prescient of STEM integration constructed upon the design options of model-eliciting actions (MEAs). The analysis group strategized a versatile and fluid skilled improvement that may (1) situate contributors’ breadth of experiences on a STEM curriculum integration continuum; (2) elicit a brand new imaginative and prescient of STEM integration by way of open-ended arithmetic issues with client-driven, real-life contexts; and (3) concentrate on making arithmetic content material specific.

Frit Kit
FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit
PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

HMGB1 Detection Kit
6010 1 kit
EUR 753.25
Description: HMGB1 Detection Kit

SEB Detection Kit
6030 1 kit
EUR 553.15
Description: SEB Detection Kit

Senescence Detection Kit
55R-1370 250 tests
EUR 712
Description: Senescence Detection Kit for use in the research laboratory

Apoptosis Detection Kit
ANXVKB-100T 100 test
EUR 464.7

Apoptosis Detection Kit
ANXVKCFB-100T 100 test
EUR 412.7

Apoptosis Detection Kit
ANXVKCFB7-100T 100 test
EUR 412.7

Apoptosis Detection Kit
ANXVKDY-100T 100 test
EUR 389.3

Apoptosis Detection Kit
ANXVKF-100T 100 test
EUR 329.5

Apoptosis Detection Kit
ANXVKF7-100T 100 test
EUR 329.5

Apoptosis Detection Kit
ANXVKPE-100T 100 test
EUR 346.4

Autophagy Detection Kit
abx299014-50tests 50 tests
EUR 523
  • Shipped within 1 week.

Autophagy Detection Kit
abx299015-200tests 200 tests
EUR 871
  • Shipped within 1 week.

Senescence Detection Kit
K2030-250 250 Staining
EUR 460

Senescence Detection Kit
K320-250
EUR 468

Formaldehyde Detection Kit
K001-F1 2x96 well plate
EUR 425

Ubiquitin Detection Kit
SKT-131-20 20 assays
EUR 495
  • The covalent attachment of ubiquitin to proteins (ubiquitination) plays a fundamental role in the regulation of cellular function through biological events including cell cycle, differentiation, immune responses, DNA repair, chromatin structure, tran
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Description: Purification Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples. in Cell Lysates, Tissue samples from all species

Glutathione Detection Kit
SKT-202-96 1 plate of 96 wells
EUR 428
  • Glutathione (L-?-glutamyl-L-cysteinylglycine
  • GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 - 10 mM (1). GSH plays a key role in many biological processes, including the synthesis of pr
  • Show more
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species

GenomeCoV19 Detection Kit
G628.v2 100 Rxns
EUR 320
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT 1 kit (10 rxn)
EUR 1110
  • Category: Cas9

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT 10 rxn
EUR 1132
  • Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT 1 Kit
EUR 805
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Rat Albumin Detection Kit
3020 1 kit
EUR 483.55
Description: Rat Albumin Detection Kit

MitoCapture Apoptosis Detection Kit
55R-1305 25 assays
EUR 277
Description: Apoptosis Kit for detection of apoptosis in the research laboratory

Luciferase Mycoplasma Detection Kit
20-abx098882
  • EUR 1233.00
  • EUR 523.00
  • EUR 648.00
  • 200rxns
  • 25 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

PCR Mycoplasma Detection Kit
abx098883-100rxns 100 rxns
EUR 537
  • Shipped within 5-10 working days.

Protein A Detection Kit
abx090813-1Kit 1 Kit
EUR 1581
  • Shipped within 5-10 working days.

Luciferase Mycoplasma Detection Kit
20-abx298016
  • EUR 1010.00
  • EUR 300.00
  • EUR 425.00
  • 200 rxns
  • 25 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

PCR Mycoplasma Detection Kit
abx298017-100rxns 100 rxns
EUR 314
  • Shipped within 5-10 working days.

MitoStep + Apoptosis Detection Kit
KMAF-100T 100 test
EUR 420.5

DNA Methylation Detection Kit
K5082100 1 kit
EUR 344

Senescence Detection Kit (Fluorometric)
K991-100
EUR 718

Thiol Fluorescent Detection Kit
K005-F1 1x96 well plate
EUR 318

Glutathione Fluorescent Detection Kit
K006-F1 1x96 well plate
EUR 354

PCR-Salmonella Detection Kit
K1447-96
EUR 620

PCR-STEC Detection Kit
K1452-96
EUR 784

PCR-Campylobacter Detection Kit
K1453-96
EUR 784

Ubiquitin Interact Detection Kit
SKT-132-20 20 assays
EUR 464
  • The covalent attachment of ubiquitin to proteins (ubiquitination) plays a fundamental role in the regulation of cellular function through biological events including cell cycle, differentiation, immune responses, DNA repair, chromatin structure, tran
  • Show more
Description: Purification Detection kit used to capture, detect, identify and characterise isolated ubiquitin binding proteins by Western blotting or proteomic analysis in samples. in Cell Lysates, Tissue samples from all species

Urine Creatinine Detection Kit
SKT-200-192 2 plates of 96 wells
EUR 342
  • Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet o
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Description: Direct Detection kit used for quantitative measuring creatinine in Urine samples from Human, Monkey, Dog, Rat

Nitric Oxide Detection Kit
SKT-212-96 2 plates of 96 wells
EUR 342
  • Nitric oxide (NO) is a diffusible, transient, reactive molecule that has physiological effects in the picomolar-to-micromolar range. Acting through soluble guanylate cyclase activation, NO is an important physiological regulator of the cardiovascular
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Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species

Hydrogen Peroxide Detection Kit
SKT-216-192 2 plates of 96 wells
EUR 441
  • In biological systems incomplete reduction of O2 during respiration produces superoxide anion (O2-·), which is spontaneously or enzymatically dismutated by superoxide dismutase to H2O2. Many cells produce low levels of O2-· and H2O2 in response to
  • Show more
Description: Direct Colorimetric assay used for quantitative measuring H2O2 in a variety of samples in Urine, Buffer, Tissue Culture Media samples from all species

Creatinine Serum Detection Kit
SKT-217-192 2 plates of 96 wells
EUR 342
  • Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet o
  • Show more
Description: Direct Colorimetric assay used to measure creatinine in Serum, Plasma samples from Human, Mouse, Rabbit, Rat, Sheep

Mycoplasma PCR Detection Kit
K1476-100 100 Rxns
EUR 298

Residual SDS Detection Kit
BSP055 100Assays
EUR 83.06
  • Product category: Molecular Biology Kits/SDS Detection

Mycoplasma Detection Kit (OKSB00010)
OKSB00010 50 Tests
EUR 382
Description: Description of target: ;Species reactivity: ;Application: PCR;Assay info: ;Sensitivity:

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
CAS9LIG-KIT 1 Kit
EUR 153
  • Category: Cas9

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN340iPS-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE620A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE622A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE624A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

Mouse CCL28 Detection Assay Kit
6724 1 kit
EUR 483.55
Description: Mouse CCL28 Detection Assay Kit

Mouse CXCL1 Detection Assay Kit
6725 1 kit
EUR 483.55
Description: Mouse CXCL1 Detection Assay Kit

Mouse CXCL14 Detection Assay Kit
6727 1 kit
EUR 483.55
Description: Mouse CXCL14 Detection Assay Kit

Human CCL5 Detection Assay Kit
6822 1 kit
EUR 483.55
Description: Human CCL5 Detection Assay Kit

Human CXCL1 Detection Assay Kit
6825 1 kit
EUR 483.55
Description: Human CXCL1 Detection Assay Kit

TUNEL FITC Apoptosis Detection Kit
A111-01 20 rxn
EUR 283

TUNEL FITC Apoptosis Detection Kit
A111-02 50 rxn
EUR 409

TUNEL FITC Apoptosis Detection Kit
A111-03 100 rxn
EUR 539

TUNEL BrightGreen Apoptosis Detection Kit
A112-01 20 rxn
EUR 306

TUNEL BrightGreen Apoptosis Detection Kit
A112-02 50 rxn
EUR 456

TUNEL BrightGreen Apoptosis Detection Kit
A112-03 100 rxn
EUR 655

TUNEL BrightRed Apoptosis Detection Kit
A113-01 20 rxn
EUR 306

TUNEL BrightRed Apoptosis Detection Kit
A113-02 50 rxn
EUR 456

TUNEL BrightRed Apoptosis Detection Kit
A113-03 100 rxn
EUR 655

Platelet Associated Immunoglobulin Detection Kit
abx290001-25assays 25 assays
EUR 968
  • Shipped within 2-3 weeks.

Platelet Associated Immunoglobulin Detection Kit
abx290001-50Assays 50 Assays
EUR 1191
  • Shipped within 2-3 weeks.

Alkaline Phosphatase Live Detection Kit
20-abx298024
  • EUR 356.00
  • EUR 509.00
  • 25 ul
  • 50 ul
  • Shipped within 5-10 working days.

CRISPR Genomic Cleavage Detection Kit
G932 25 Reactions
EUR 205

Cholesterol Detection Kit (cell-based)
K587-100
EUR 294

Copper Detection Assay Kit (Fluorometric)
K899-100
EUR 457

Mitochondrial Apoptosis Detection Fluorometric Kit
K2097-100 100 assays
EUR 502

Mitochondrial Apoptosis Detection Fluorometric Kit
K2097-25 25 assays
EUR 238

CRISPR Genomic Cleavage Detection Kit
K1250-25
EUR 403

PCR-Salmonella-Listeria Detection Kit
K1448-96
EUR 784

PCR-Listeria monocytogenes Detection Kit
K1449-96
EUR 648

PCR-Legionella spp Detection Kit
K1450-96
EUR 648

PCRAgH5 AIV Detection Test Kit
PD55-02 1 kit
EUR 902.47
Description: Please check the datasheet of PCRAgH5 AIV Detection Test Kit before using the test.

Blood Urea Nitrogen Detection Kit
SKT-213-192 2 plates of 96 wells
EUR 428
  • Urea is a by-product of protein metabolism by the liver, and is therefore removed from the blood by the kidneys. Urea freely filters through the glomerulous, but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate
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Description: Direct Colorimetric assay used for quantitative measuring urea nitrogen in a variety of samples in Serum, Plasma, Urine, Saliva, Tissue Culture Media samples from all species

vWF Acty. Kit
ABP-ACT-KIT 12 x 8 microwells
EUR 428

vWF Ant. Kit
ABP-TOT-KIT 12 x 8 microwells
EUR 394

Monoclonal Anti-Vesicular Stomatitis Virus Glycoprotein (VSV-G)-Cy conjugate for Immunofluorescence
VSV11-Cy 100 ug
EUR 347

Qualitative evaluation of participant discussions and written reflections from a four-day summer season institute signifies that the each day tailoring of the skilled improvement design supported an evolving participant envisioning of STEM integration. Opportunities to interact with MEAs as learners, distinction MEAs with problem-based studying and draw from MEA design options to change current curricular duties allowed contributors to assume extra broadly about arithmetic content material inside STEM integration. Participants communicated a readiness to make use of MEAs as a car for Okay-6 STEM integration which maintains an necessary grounding in the educating realities of grade-level requirements and standardized take a look at preparation.

They additionally acknowledged the necessity for ongoing help as they thought of the challenges of curricular pacing and administrative expectation.The researchers continued to help the varsity division throughout month-to-month tutorial-year skilled improvement classes because the lecturers and coaches created and enacted prototype classes. Their shared funding in constructing STEM integration capability with a selected concentrate on arithmetic content material can provide a mannequin for STEM integration utilizing MEAs that challenges one-size-fits-all skilled improvement, encourages STEM tutorial management, and promotes mathematical readiness for STEM citizenship and careers.